FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20827803
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC324870.1559790055629007No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC287610.13808945667481107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT53250.057.2792935
TGCGACA53200.057.229534
CTGCGAC60350.051.1575663
CGACATC64900.046.923616
GCTGCGA71550.043.415212
GGCTGCG97100.033.2802541
TTGATCG59700.026.01615122-23
CTAGGCG130300.025.9969987
TAGGCGG132100.025.4989018
TGATCGC63400.024.42299824-25
GATCGCC66800.023.21546724-25
TCGCCAG69500.022.55276726-27
GCTAGGC157200.021.8204026
ATTCGGC72400.021.09235438-39
ATCGCCA77500.020.07152226-27
TTCGGCT77750.019.73273840-41
TGATTCG78000.019.73001336-37
GACATCT158350.019.6817577
GGCTAGG181600.019.4120035
CGGTCGA92050.018.395968-69
Produced by FastQC (version 0.11.5)