FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14005624
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT20500.050.0318345
TGCGACA21650.046.7151154
CTGCGAC23550.044.5591663
GCTGCGA26300.039.7195362
CGACATC26400.038.1307536
TAACGCG9000.029.81057270-71
GGCTGCG37600.028.804751
ACGCGAC10550.027.23093872-73
CTAGGCG50350.026.783197
AACGCGA11250.026.16998770-71
TAGGCGG51650.026.0165848
GGTGTGC74450.024.044747
GTCGGGT26150.023.2419418
GTAACGC11800.023.13913368-69
CGACCGA12550.022.32419274-75
CGCGACC13000.021.91631972-73
GCGACCG13050.021.65079974-75
CGCTTCG16550.021.46490542-43
CGAAGCG17100.021.1044948-49
GTGTGCT114750.021.1033618
Produced by FastQC (version 0.11.5)