FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15457086
Sequences flagged as poor quality0
Sequence length41
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN350920.22702856152835016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG14950.022.2302222
CGCGTTA7250.021.9574935
CGATCGG6150.020.1792287
ACGGTAC15450.019.70230532
CCTACGT17400.018.9989623
TCGATCG5700.018.7079856
TCGCCCG6550.018.6966133
GGGCGAC17200.018.6537551
TACGTTC18350.018.01559825
CGGTCGC8550.018.00581731
CTTACGG17600.017.99046529
GAACGCA10050.017.9287735
TCGCGTT8550.017.8015534
CCTTACG17650.017.54293328
GTCGCGT8800.017.49468433
CGGCCGA7200.017.49053811
TCGGCCG7200.017.49002810
CGGTACT17850.017.34767233
TACGGTA17950.017.25063531
CTACGTT19350.017.08439324
Produced by FastQC (version 0.11.5)