FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17088650
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT19850.032.794431
CCGATCG38100.028.75698386-87
CGGGGAT72650.027.9214828
CCCGTTA15800.027.2216173
CCCCCGT29450.027.1516461
TCCGACC21000.026.938239
CGTCCGT7350.026.500441
ATAGCGC42850.026.04892294-95
TCGGGGA79400.025.975597
GTCCGTT19750.025.9753252
CGATCGG44050.025.66816986-87
CCCCGTT33450.025.4062542
CCGTTAT15550.025.3272174
TATCGGG43100.025.0085725
CCGTTAA16300.024.7976914
GCCCCGT28000.024.5303421
CGGTCGA13300.024.03427738-39
GGGCCGT25250.023.344971
CCCCGAA31100.023.327092
TTCCGAC24850.023.3227448
Produced by FastQC (version 0.11.5)