FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073953
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC24850.045.9642339
TTCCGAC27350.042.3896458
TCACCCC99600.038.597099
TTCACCC105650.036.9687088
ATAGCGC52800.035.00762694-95
CCGATCG58700.033.2985186-87
GTTCACC117500.032.6741567
ACCCGAT61850.031.9884884-85
CCGTTAA7950.031.8060864
CGGGGAT48000.031.5602668
GATCGGC63200.031.41328888-89
CGATCGG60500.031.34051186-87
CGGCATA60650.031.15562690-91
CGGTTCA59500.031.1354945
TTTCCGA38000.030.5540147
CATAGCG63700.030.30288794-95
GTTCCGT15200.030.2330551
ATCGGCA65250.029.31407288-89
CACCCGA65050.029.09352782-83
CCCGATC65550.029.00413384-85
Produced by FastQC (version 0.11.5)