FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13910023
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC51800.042.2252588
TCCGACC54600.039.8104679
GTAGCAA115800.036.5235379
TCGCGCG34300.036.4172947
TTTCCGA63450.034.7672357
TAGTAGC121450.034.697327
ATTGCGC32000.033.889639
CCGTTTC70700.032.919074
AGTACGA34950.032.3782239
CCGTTTA25800.032.3216554
TCCGTTT68700.032.157413
GTTTCCG63450.031.1942836
CTCCGTT59550.031.0811842
TCAGTCG76900.030.73156784-85
CATTGCG35750.030.7230268
AGTAGCA138950.030.4650488
ATAGCGC66600.030.18661394-95
CGTTTCC68600.029.9471575
CCGATCG74800.029.90468486-87
AAACGAG88950.029.90425522-23
Produced by FastQC (version 0.11.5)