FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804100
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT11300236.7246862372873295No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG5557523.307240494879226No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4604172.7399087127546253No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3707292.2061818246737404No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG3403022.02511291887099No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2788891.659648538154379No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT2430081.446123267535899No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1254830.7467403788361174No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1186690.706190751066704No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1026880.6110889604322755No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC998790.5943728018757327No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA956020.5689206800721253No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG878810.5229735600240417No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG864430.5144161246362495No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC745100.44340369314631545No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC724690.43125784778714715No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA700280.41673163097101296No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA696070.4142262900125564No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC693890.41292898756851004No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT621430.3698085586255735No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA606880.3611499574508602No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA550330.3274974559780054No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA537830.3200587951749871No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT533900.3177200802185181No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG525410.31266774180110807No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA516850.3075737468832011No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG512930.3052409828553746No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA505460.3007956391594908No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA503710.2997542266470683No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA499720.29737980611874487No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT498380.29658238168066126No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC470780.28015781862759687No Hit
TACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCT456150.27145161002374424No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC426440.2537714010271303No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG388270.23105670639903356No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA387530.23061633767949488No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC363880.21654239144018425No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC363320.21620913943620904No Hit
TGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGT357600.21280520825274785No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC347890.2070268565409632No Hit
ACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGG341600.20328372242488438No Hit
ACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC341140.2030099797073333No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG323910.19275652965645287No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC319730.19026904148392357No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG312950.18623431186436643No Hit
ACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGA306100.1821579257443124No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC301220.17925387256681405No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT296300.17632601567474604No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG293820.1748501853714272No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG288770.1718449664070078No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG285810.17008349152885308No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT281520.16753054314125718No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC280600.16698305770615504No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT280210.16675097148910087No Hit
CCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGAC278390.1656679024761814No Hit
TCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTG276200.1643646491034926No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC275250.16379931088246322No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA271000.161270166209437No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG270020.16068697520248035No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT262710.15633684636487524No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG262190.15602739807546967No Hit
GACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG261780.15578341000113066No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA259200.1542480704113877No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT256080.15239138067495434No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG254010.1511595384459745No Hit
TCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCT253150.15064775858272683No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT251970.1499455490029219No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA246830.14688677168072078No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG246400.14663088174909694No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA242770.14447069465190043No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG239260.14238191869841288No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAAT238150.14172136561910487No Hit
AACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCC226590.13484209210847353No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC219570.13066454020149845No Hit
AACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGT218420.12998018340762077No Hit
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC217400.12937318868609446No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT217210.12926012104188858No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG217120.12920656268410685No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA214150.1274391368773097No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT213200.12687379865628032No Hit
CATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGG209770.12483263013193209No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT209640.1247552680595807No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA209110.12443986884153271No Hit
AATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCT209050.12440416326967824No Hit
AGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTA208600.12413637148076957No Hit
GACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACAT201360.11982789914366136No Hit
GACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTG197790.11770341761831934No Hit
GCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT196540.1169595515380175No Hit
CCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGT196510.11694169875209026No Hit
GGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCAT196230.11677507275010265No Hit
ACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGC193870.11537065359049278No Hit
ACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTC193510.11515642015936588No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA192160.11435304479263989No Hit
CCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG190480.11335328878071423No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG190140.11315095720687213No Hit
TCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG188290.11205003540802543No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA184800.1099731613118227No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG184180.109604203735993No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG183970.1094792342345023No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTT183700.1093185591611571No Hit
CACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA183530.10921739337423604No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG182410.10855088936628562No Hit
TGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGA181430.10796769835932898No Hit
CCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAAC178470.10620622348117424No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG175780.10460542367636469No Hit
TTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGA174340.10374848995185698No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT173010.10295701644241584No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC169820.10105867020548556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG5450.076.8611
GCGACAT9050.073.502725
TGCGACA9650.068.930354
ACGTGGC10150.065.188151
GCACGAC9150.062.428091
GTAACCG3350.059.679391
CTGCGAC11700.057.2769783
TAACCGC19550.055.9716152
GACTCCC2061300.055.9295437
ACTCCCT2061350.055.8613748
AACCCGA2064000.055.8348052
CTCCCTT2067150.055.6793679
ACCCGAC2076300.055.621813
CCCGACT2087200.055.304774
CCGACTC2088750.055.2359665
CGACTCC2087350.055.2231256
GCAACAC16550.050.6214451
TATACCG2650.048.4995571
GTAGCAA60650.048.4671259
AGTAGCA61400.047.9524428
Produced by FastQC (version 0.11.5)