FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804100
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG4979522.963276819347659No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA3267771.9446266089823316No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA2107511.2541641623175297No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1989861.1841514868395213No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1959691.1661975351253562No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1189650.7079522259448587No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1153080.6861896798995484No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG1146530.6822918216387667No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1088630.6478359447991859No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1034410.6155700097000137No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA940760.5598395629638004No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA822560.4894995864104594No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG815060.4850363899286484No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG803460.4781333127034474No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA798950.47544944388571836No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG762790.453930885914747No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA735420.4376431942204581No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG717710.42710409959474177No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC626010.3725340839437994No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA592980.35287816663790383No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA533120.31725590778440976No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA519430.3091090864729441No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC479600.2854065376902065No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA467610.2782713742479514No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC457570.2722966418909671No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG426890.254039192816039No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC407630.24257770425074832No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG405540.2413339601644837No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA404330.2406138977987515No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA394240.2346094107985551No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA390080.2321338244833106No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT381360.22694461470712507No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA373390.22220172457912057No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG372020.22138644735510976No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG362150.2155128807850465No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC361030.21484637677709606No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA354910.2112044084479383No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA352990.21006183014859467No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT337990.2011354371849727No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC323620.19258395272582285No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG305860.18201510345689445No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG290450.17284472241893348No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA287270.17095232711064562No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG283660.16880404187073392No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC277770.16529894490035169No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG274570.163394647734779No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG270820.1611630494938735No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG268840.1599847656226754No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA265550.158026910099321No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG264960.15767580530941855No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG264310.15728899494766158No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA263200.15662844186835356No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG254090.1512071458751138No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC251790.14983843228735844No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG250990.14936235799596526No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA249470.14845781684231824No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT249190.14829119084033063No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA247590.1473390422575443No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA247250.1471367106837022No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC242830.1445064002237549No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA239830.1427211216310305No Hit
CGACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG238410.14187608976380767No Hit
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA237930.14159044518897174No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG235660.14023958438714362No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA232860.1385733243672675No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC232270.13822221957736505No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG226710.13491350325218252No Hit
CGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA224530.1336162008081361No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA219400.13056337441457738No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA212390.12639177343624472No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG209520.12468385691587172No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG209030.1243922614123934No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG208500.12407686219434544No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT208190.12389238340643058No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA207720.12361268976023708No Hit
TGTGAGGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA207430.12344011282960705No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC206140.12267244303473557No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA203870.12132158223290745No Hit
TTTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG202060.1202444641486304No Hit
TTGTGGAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA201400.11985170285823102No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC200150.1191078367779292No Hit
CTTCGTTGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT197620.1176022518313983No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT197090.11728685261335031No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC195380.1162692438154974No Hit
GTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC193440.11511476365886897No Hit
TCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAG193300.11503145065787515No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA191850.11416856600472503No Hit
CGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG189280.11263917734362447No Hit
CGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG187740.11172273433269261No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG187370.11150254997292328No Hit
TGTGGAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAA186210.11081224225040318No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT185100.11015168917109515No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA181650.1080986187894621No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC181380.1079379437161169No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG178430.10618241976660457No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC177850.10583726590534452No Hit
GGCGCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC176000.1047363441064978No Hit
TTGTGTAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC175600.10449830696080123No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG173560.10328431751774865No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC173400.10318910265947No Hit
GTGGGTTTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC172450.10262376443844062No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA170120.101237198064758No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA168080.10002320862170541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA11200.089.131193
TGGTGTT230300.088.4455951
GTGCTAG155850.087.295383
GGTGCTA152600.087.191252
TGCTAGT162850.086.637234
TGATAGT300650.086.2984
CGACCTA30750.086.105211
GGTGTTA279500.084.884872
TTCGACG13050.084.143551
TGGTGCT141650.083.963411
GATAGTA356650.082.930865
TACGGTG26050.081.752063
GTACGGT25750.081.596132
CGGTGAT24400.081.0443651
GACCTAT38900.080.8970952
CGGTGTT24050.079.8519741
CCGGTGC52000.079.166783
CTTCGCT18950.078.013641
CTTCGTT28250.077.73971
GGTGATA307900.077.5647662
Produced by FastQC (version 0.11.5)