FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17085017
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA27650.042.6033444
GCGACAT32000.037.1088185
CTGCGAC34800.033.4405943
CGACATC38900.030.1602176
GCTGCGA42150.028.2855432
ACTCGGG83050.027.2362731
CTCGGGA94750.023.8625092
TCGGGAG111000.020.454653
GGCTGCG70150.018.2901151
CGCTTCG24300.017.78764342-43
GGTGTGC94450.017.6016437
GTGTGCT162100.016.5264438
TTGATCG38250.016.4541722-23
CGAAGCG24900.016.30985348-49
TAGCCCC43250.015.4922091
TGATCGC39750.015.17587324-25
CGGGAGG164250.014.4594494
TCGGTCG35650.014.39000260-61
GCGCGGT21100.013.7320226
TCGCCAG43800.013.60987726-27
Produced by FastQC (version 0.11.5)