FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17085017
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC36100.050.1547559
TTCCGAC40950.044.6957558
ATAGCGC77950.033.83282594-95
CCGATCG77700.033.6209786-87
CGATCGG84250.031.67214686-87
ACCCGAT83800.031.34865884-85
CCCCGTT38100.031.3452592
TTTCCGA59500.031.155997
CCGTTTA26750.030.6698934
TCACCCC162850.030.6524859
CGGCATA88900.030.41199590-91
GCCCCGT29350.030.2723311
CACCCGA89700.030.22566882-83
GATCGGC89250.029.9011488-89
CCCGTTA19600.029.4056133
CGGTTCA79950.029.3559465
ATCGGCA92900.029.35464988-89
CCGTTTC63400.029.3205914
GTTCCGT20150.029.3110031
CCCGATC91450.029.28802984-85
Produced by FastQC (version 0.11.5)