FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15982492
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC35400.046.243949
TTCCGAC38800.043.0215268
TCGATCG17600.033.78766
CCGATCG69550.033.09177486-87
ATAGCGC66700.032.5559394-95
TTTCCGA54200.031.2557077
CCCGTTA16350.031.0397973
ACCCGAT74700.030.93581284-85
GTCCCGT19150.030.5289271
CTCCGTT46700.028.9798452
CCCCGTT36400.028.870982
GTTTCCG51500.028.7675346
CATAGCG80850.028.3454994-95
CGGCATA77900.028.1455590-91
GCTCCGT45250.027.9932861
CCGTTTC61800.027.9545154
TCACCCC158250.027.9463779
TCCGTTT62800.027.672063
GTTCCGT22750.027.5013451
CCCGATC80950.027.08994584-85
Produced by FastQC (version 0.11.5)