FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24376348
Sequences flagged as poor quality0
Sequence length41
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN543660.2230276659982045No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267760.10984418174535414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC43650.024.2021799
CGACCTG44900.023.33521311
CCCTACG35700.023.2230422
CCGACCT45300.023.05136910
CGATCGG16600.022.4309717
TTCCGAC48650.021.8877588
TCGATCG14900.021.8244116
CCTACGT38350.021.2984923
TCCCCCG135800.020.49412535
TCGCCCG18050.020.3530633
TACGTTC41100.019.74602725
GTCCCCC143600.019.47943134
GGCAACC146250.019.27871522
TGGGCCG54750.019.13554816
ACGGTAC32150.019.0990232
CGGCCGA19250.019.08182111
CTACGTT42900.019.08076124
GGTCCCC149100.018.74937233
GATCGGC20250.018.6507178
GCTCCGT43250.018.585221
Produced by FastQC (version 0.11.5)