FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15970234
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT312560.1957141016218047No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT229590.14376119974196996No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC179410.11234024498326073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA41050.047.903934
GCGACAT43250.045.5770265
CTGCGAC51300.038.702853
GCTGCGA57250.034.763752
CGACATC59500.033.2093476
ACTCGGG183600.032.5326421
CTCGGGA198350.030.365132
TCGGGAG214550.028.1163673
GCGCGGT45950.024.9123976
GGCTGCG85500.024.398051
CGGGAGG285850.021.7845464
CGCGGTG58450.019.5034187
GGTGTGC86050.019.2645367
GTGTGCT137100.018.95098
TTGATCG53900.018.81502222-23
TCGCACG14450.018.40772442-43
TGATCGC56100.017.82291224-25
GGAGGCT414850.017.586696
GGGAGGC406300.017.4774655
GGGCGCG68300.017.1775134
Produced by FastQC (version 0.11.5)