FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15970234
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA220660.13816954717131882No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG198580.12434382614556556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC85800.060.449459
TTCCGAC90300.057.7854548
TTTCCGA109050.047.67747
TCACCCC343100.046.869359
TTCACCC346500.046.060918
GTTCACC346050.045.1334727
GTTTCCG100600.043.9376956
CGTTTCC99700.042.980265
CCGTTTC102700.041.1682744
CTCCGTT78250.041.086422
TCCGTTT100250.040.3076253
GTCGGTT26900.038.616213
GGTTCAC277950.037.8971826
GCTCCGT76250.037.7959631
ATAGCGC216050.037.6182294-95
CGGTTCA210500.036.6918075
CCGATCG235250.035.25603586-87
ACCCGAT239550.034.69936884-85
CGGCATA241350.034.24814690-91
CGATCGG244350.033.92393586-87
Produced by FastQC (version 0.11.5)