FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13992377
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC191200.1366458322270762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA38850.057.0964474
GCGACAT40150.055.2475475
CTGCGAC46600.048.2123643
CGACATC49500.044.9078566
GCTGCGA51050.044.19662
GGCTGCG73150.031.6361871
TTGATCG47550.023.52538922-23
GGTGTGC92000.023.4912017
TGATCGC48500.023.01540824-25
ACTCGGG70100.022.7766971
GTGTGCT145750.020.9222378
GACATCT109600.020.6723337
TCGCCAG56150.020.1330926-27
ATTCGGC57000.019.33280238-39
CTCGGGA81750.019.1741942
TGATTCG57850.018.96663536-37
GATCGCC59000.018.83893624-25
TTCGGCT59100.018.7262240-41
CGCTTCG20450.017.65232742-43
ACATCTG129300.017.5594548
Produced by FastQC (version 0.11.5)