FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15252602
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC31200.035.2672739
TAGTAGC91050.032.313257
ATAGCGC55100.029.4322394-95
CCGATCG55800.029.23481886-87
TTCCGAC38550.029.0459758
CAGTACG33200.028.9956978
ACCCGAT58900.028.01190484-85
GTAGCAA107050.027.8793189
AGTACGA36050.027.0382659
TCAGTCG66850.026.99986584-85
CGAGAAC68950.026.94732524-25
CGGCATA60250.026.66058790-91
TATCGGG43450.026.388685
TCTATCG40950.026.2495373
CAAACGA72000.026.21506320-21
ACGAGAA74200.026.09200124-25
TCACCCC124750.026.0180249
CCGTTTA26850.025.9271074
AAACGAG74500.025.7591522-23
CAGTCGG72250.025.75055384-85
Produced by FastQC (version 0.11.5)