FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23348681
Sequences flagged as poor quality0
Sequence length41
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN516820.22134869203104024No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363790.15580751649311583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC21850.024.49585523
TAACGCA22250.023.81979824
CCCTACG42150.023.19771822
CCTACGT43350.022.4744323
TCCGACC31450.022.3549149
CGATCGG10850.021.1050427
CGACCTG33650.020.84404211
TCCCCCG94800.020.79664435
AACGCAG26800.020.68962925
TACGTTC46950.020.60239625
CTACGTT47300.020.59773424
CGGTAAC27150.019.84295521
CCGACCT35700.019.84207310
GACTACC49300.019.72630917
ATCGCGA12150.019.72272719
GTACGAA27650.019.35648510
TCGCGAA12400.019.32492320
ACTACCC51200.019.26778418
GGTAACG27950.019.21266422
GGCAACC107400.019.0551622
Produced by FastQC (version 0.11.5)