FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570263
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT751510.8768809078554531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327500.38213529736485335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303800.3544815369143281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156780.1829348760942342No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG134820.1573113917274184No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA110200.12858415196826514No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC98670.11513065585035137No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC97040.1132287305535431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.086.92121
GTATCAA294750.072.870521
ATCAACG367200.058.087923
CAACGCA370800.057.585775
TCAACGC371950.057.3461044
AACGCAG390050.054.7945256
ACGCAGA419250.050.9604387
CGCAGAG420100.050.8573238
TATCAAC439550.048.6619572
GCAGAGT451800.047.3021439
GCAGCGC14550.044.9550678
TGCAGCG14400.043.358657
TGGTCCT36550.032.2209475
AGAGTAC492050.031.6984510-11
CAGAGTA478750.030.6041910-11
TCTGTCG10050.029.583738
GTACTTT597250.029.1603761
TGGTATC29800.028.542892
GTGGTAT32850.027.522411
AGTACTT491450.026.74607812-13
Produced by FastQC (version 0.11.5)