FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570263
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG149950.1749654590530069No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA108950.12712562029893365No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC108600.12671723143152083No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC92090.10745294514299035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA71900.049.5984085
CGCAGAG76000.046.891188
ACGCAGA76500.046.5061157
TCAACGC78250.045.4974064
ATCAACG78600.045.0675773
GCAGCGC13450.044.2339448
GTATCAA85900.043.6804731
GGTATCA30700.042.2919851
AACGCAG86100.041.3195576
GCAGAGT95650.037.3830769
TGCAGCG14600.036.266657
TGGTCCT35950.032.6239935
GTCTCGC9100.029.4517461
TCTGTCG8550.026.4420668
GAGTACG86350.025.05152712-13
GACGACC7300.024.842407118-119
TAGCGTG7950.024.6963259
AATGCGT41250.024.2294186
AGTACGG103550.023.96503412-13
TACGGGT15750.023.8138624
Produced by FastQC (version 0.11.5)