FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13578310
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC294560.2169342134625001No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC235300.173291079670445No Hit
GGCAGTATGATGGCCTAGATGCAGAGAAAACAGCTCCTTGGTGAATTGAT142650.10505725675728422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA34000.067.59884
GCGACAT35200.065.157715
CTGCGAC37200.062.042123
CGACATC37400.061.5695346
GCTGCGA42700.054.161962
GGCTGCG56000.042.160491
CTAGGCG87300.033.773277
TAGGCGG88950.033.0934078
TTGATCG40600.028.29756522-23
GCTAGGC110700.027.1917636
ATTCGGC43250.026.68636738-39
TCGCCAG44000.026.49174526-27
TGATCGC44100.026.05412524-25
CGGTCGA56450.025.96826768-69
TTCGGCT45000.025.65059740-41
TGATTCG45650.025.64454836-37
CCGATAG47600.025.54417890-91
GATCGCC45750.025.27013624-25
TCGGTCG57900.024.98966668-69
TCGACCG5900.024.9518643
Produced by FastQC (version 0.11.5)