FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13578310
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN422620.31124639222406913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC20000.038.2584429
CCCCGTT34750.034.5694962
GCCCCGT23350.033.818841
GTTCCGT15950.032.8072281
GTCCCGT15200.031.9222951
CCCGTTT44600.031.7491663
TTCCGAC24550.030.9730388
CCCCCGT26150.030.3796181
CCCGTTA17900.029.7314363
CCGATCG19900.029.71179486-87
CCGTTAT15700.029.3577654
CCCCGAA26900.029.3010462
CTCCGTT33150.028.6466462
CCCCGTA16750.028.063882
CCGTTTC44700.027.8512484
CGGGGAT65850.027.496958
CCGTTTA22100.027.3062694
GCCCCGA20350.027.116341
TCGGGGA67200.027.082087
GGCCCGT18850.026.7505071
Produced by FastQC (version 0.11.5)