FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12464401
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3843623.083678068444685No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG3301952.649104437509673No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1626111.3046034061323926No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1268761.0179069174683966No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1252861.005150588463898No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1159190.9300005672153839No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG744710.5974695454679291No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC672370.5394322599216761No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT529090.4244808876094407No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC478950.38425432557890266No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG422570.3390215061277313No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC416160.3338788602837794No Hit
CAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAA386990.3104762114120045No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA369220.2962196097510021No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA367250.29463910861019316No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG343440.27553670649716744No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG333250.26736142394648565No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC331800.26619811092406287No Hit
TAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTT306820.24615703554466836No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC273520.21944095027109606No Hit
ACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCA257090.20625941029978095No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC253380.20328293353206467No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC211920.17002020393920253No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA208050.16691536159659817No Hit
GGGTTGTTTCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCC206530.16569588863516185No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC205950.16523056342619272No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT181420.14555051622617082No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT181240.1454061049544218No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTC179880.1443149975678735No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC179720.14418663199298548No Hit
ATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATT178070.1428628620019526No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT173610.13928467160194863No Hit
CAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGG173540.1392285116629351No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT167810.13463141951225735No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC167010.13398959163781718No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG165770.1329947584324349No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC165410.1327059358889368No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT157480.12634381708354858No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG149780.12016622379206188No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC148700.11929975616156765No Hit
CACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCCCGA148190.11889059089161204No Hit
GAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGA147510.11834503719833789No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA145820.11698917581358301No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC144820.1161868909705328No Hit
TAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCG144110.11561726873196715No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG143210.11489521237322195No Hit
CCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGA142310.11417315601447676No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT142300.11416513316604623No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC142020.11394049340999218No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC140990.11311414002165047No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC140320.1125766091768068No Hit
AGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGT139870.1122155809974342No Hit
CGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCA139120.11161386736514656No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA136020.10912678435169086No Hit
AGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCT135610.10879784756604029No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT135230.1084929793256812No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG132750.10650331291491666No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT132110.1059898506153645No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA129920.10423284680908453No Hit
GACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGT127240.10208272342970995No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG127220.10206667773284893No Hit
AGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTT126570.10154519258486629No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA125610.10077499913553808No Hit
CCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC125550.10072686204495507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACG111550.070.2123957
TGCGACA19500.066.4954
GCGACAT20000.065.308385
GCTTGGA125050.063.3926435
CTGCGAC20700.062.6402173
GGACGCG130950.060.207859
CTTGGAC136300.057.637136
TGCTTGG138900.057.0709464
ACTCCCT683600.056.30678
GCAACGC12250.055.8482171
CGACTCC695150.055.446326
GCTGCGA24100.054.985272
CTCCCTT701950.054.6302389
GCTCCCG622550.053.222468
CCGACTC728050.052.980315
CCCGACT741650.052.2643244
GGGCTCC633350.052.2624056
ACCCGAC745000.051.7997973
AGTTTCG106600.050.615548
GTTTCGT107200.050.5966579
Produced by FastQC (version 0.11.5)