FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10109116
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC282050.279005602468109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG10600.053.3016556
CGACGCT48000.052.127758
GACGCTC54300.044.24039
CCGACGC30800.044.0874447
TCGACGC10200.043.7549557
CTAGGCG65400.043.7037127
GTCGACG4900.043.6029666
TAGGCGG65650.043.4649668
GCCGACG15900.042.1038256
GCGACGC22000.039.0620887
GCTAGGC80450.035.8230256
GCGACAT14900.035.694055
TGCGACA14850.034.2216454
GTGCGAC11750.033.9473155
CTCGACG6600.033.810656
GGCTAGG90550.032.880865
AGGCGGG98200.029.2490189
CTGCGAC18150.028.7845613
TCCGATC49500.028.14989316-17
GGCTGGC104500.028.140221
Produced by FastQC (version 0.11.5)