FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10109116
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN317920.3144884280682901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC17300.035.4333429
TTCCGAC18250.033.3253368
CCGATCG33750.030.88304586-87
ATAGCGC33350.030.59311994-95
GTTCCGT14800.030.2247181
CGATCGG34800.029.74654886-87
CGGTGTG21550.029.3232635
GATCGGC36300.029.23682888-89
CCCGTTA9800.029.0891483
GGTCCGT9850.028.0212761
ACCCGAT37350.027.97153384-85
CGGGGAT53550.027.9497878
ATCGGCA37950.027.71540888-89
CGGTTCA36650.027.483365
CCCCGAA20350.027.2993762
CGGCATA38250.027.0627290-91
GCCCCGT17000.026.5932121
CTACCGA5600.026.3432771
CATAGCG39750.026.32711294-95
GCTCCGT19300.026.1363351
Produced by FastQC (version 0.11.5)