FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11445725
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG122570.1070880175786156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC43550.051.8315438
TCCGACC46450.048.714299
TTTCCGA54250.042.125687
TCCGTTT56550.038.2179643
GTTTCCG54400.037.236756
CCGTTTC59000.036.956644
TCGCGCG56900.036.5200427
CGTTTCC57850.036.0663225
CTCCGTT48900.035.3315732
ATTGCGC19950.034.7356389
GCTCCGT47200.034.6029781
CCTCGCG58500.031.7206335
CGCGCGT66850.031.0168748
CGATCGG39750.030.39759386-87
GCCCCGT33300.030.3892631
GCGCGTC69000.030.061079
CCGATCG42850.029.48021586-87
ACCCGAT43850.028.91439284-85
ATAGCGC39450.028.3927594-95
TCACCCC144150.028.1244899
Produced by FastQC (version 0.11.5)