FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17483944
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA306300.17518930511330852No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC234400.13406586065478132No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA203410.11634102694449261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC16700.050.9062046
GTACCGA16850.049.9094855
GTCGACG6950.041.7032284
CACCGTC109750.039.0348329
TACCCGT15450.038.1342621
CGACTGA184850.038.052749
CCACCGT112250.037.8680848
CCGTTTA44250.037.4745834
ACCGACT187650.037.2306637
CGTGTCG18800.036.899221
TCCGGAT91950.036.8793378
CCGTCGA7350.036.8472182
CCGACTG192950.036.429538
TCGGTAT20150.036.3124355
CGTCGAC8200.035.3454253
CGCTCCG28300.033.9136625
TGACGGC24700.033.649246
CGACGCG7750.033.1067664
GCCACCG129550.032.8478747
CTCCGGA101700.032.363017
Produced by FastQC (version 0.11.5)