FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16119578
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG868720.5389222968492103No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG371730.2306077739752244No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA346990.21525997764953894No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG309310.1918846758891579No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG257470.15972502505958902No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA183660.11393598517281282No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG168600.10459330883227838No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA167230.10374341065256173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC675500.049.7279977
GTAGCAA698350.048.885059
AGTAGCA695550.048.841158
GGTGTTA110400.042.140822
GTAGTAG520200.041.3079456
GATAGTA97450.041.0098955
GGTAGTA425850.039.2901655
TGCTAGT79850.038.2082864
GGTGGTA301300.037.979282
GGTGCTA81050.037.7005162
GTGCTAG81700.036.760753
GTGATAG96550.036.1271063
TTCGACG5850.035.7430461
ATAGTAG127800.035.642586
GCTAGTA87150.034.9514475
AAACGAG648150.034.56868422-23
ACGAGAA650350.034.42549524-25
AAGGCCG649850.034.4077336-37
CGAGAAC642650.034.3835624-25
TCAGTCG609000.034.35952484-85
Produced by FastQC (version 0.11.5)