FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18821981
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG529000.2810543693567643No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT458160.24341752337333675No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT342260.18184058309271484No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG291660.15495712167598086No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC222410.11816503268173525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTC98350.046.723125
CGACTCC101300.045.5484736
AACCCGA103900.044.6404232
CCCGACT106800.043.737574
ACCCGAC115650.040.021173
GAACCCG134650.036.391921
CTCACGT165700.033.317469
GACTCCC144950.031.864837
TCGATCG77400.029.86788416-17
GCTCACG186150.029.5809388
GTAGCAA150200.027.87499
ACTCCCT171650.027.2401458
GTAGTAG139850.027.2225556
CGGAACG82750.027.1982956-57
AGGGGCG51200.027.1653087
GTCGGTC59450.027.07231386-87
CGGTCTA89700.027.0424126
CCGACTG137250.026.8044138
TTCGATC87650.026.61880514-15
CGACGGT92000.026.3155943
Produced by FastQC (version 0.11.5)