FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18821981
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283580.15066426854856563No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG280730.14915008149248477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC241600.040.9879267
GTAGCAA258600.038.791259
AGTAGCA274350.036.528678
CGAGAAC202250.031.8220724-25
AAGGCCG206850.031.74291836-37
AAACGAG209550.031.48557922-23
CAGTCGG190100.031.47759684-85
CGAAGTG200100.031.42102240-41
TCAGTCG191700.031.40060484-85
CAAACGA205650.031.33246220-21
ACGAGAA214100.030.66022324-25
CGACTGA69150.030.1822859
GTCGGTC194350.029.98346586-87
GTAGTAG223600.029.638396
AGTCGGT202350.029.5839986-87
GACCGAC54950.029.328896
GGTAGTA186300.029.1641185
TCGGTCC201900.029.15650488-89
GGCCGAA223250.029.1540938-39
TCAAACG227400.029.02546120-21
Produced by FastQC (version 0.11.5)