FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915015
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455250.658349981887241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254400.3678950804878948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206340.2983941466504411No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112370.16250145516676393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75900.074.095511
GTATCAA193150.060.6362341
ATCAACG239200.051.6979943
CAACGCA239300.051.6262745
TCAACGC241750.051.2261544
AACGCAG252150.049.200256
CGCAGAG280000.044.305628
ACGCAGA280450.044.2563867
TATCAAC290700.042.6837842
GCAGAGT311450.039.831679
GTCTCGC10300.037.5558851
CTCGCTC11700.036.112853
TCTGTCG11750.035.4463048
GCAGCGC8150.035.042458
CGCTCTG12300.032.899555
AGCGCGA953.284241E-431.3219131
CGTCAAT4350.030.0921766
TCTCGCT14450.029.6528572
TGCAGCG9750.029.2923157
GTACTTT450700.028.8777331
Produced by FastQC (version 0.11.5)