FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915015
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11500.042.9892731
CTCGCTC11600.040.5136643
CAACGCA39450.039.9561965
CGCTCTG12050.039.49015
CGCAGAG41250.037.9401868
TCTCGCT12950.037.2089732
ACGCAGA42850.036.937197
TCTGTCG12800.035.7971468
TCAACGC47100.034.092024
ATCAACG48250.033.165563
GCAGCGC6950.032.536178
AACGCAG50300.031.5828426
GTATCAA60550.028.724241
GCAGAGT62700.024.7749619
CGCGGCA2900.024.65212652-53
GTACGGG57350.023.6799761
GCTCTAG17250.022.7894941
GGACCGT2352.9135663E-722.7867586
GAGTACG45500.022.7629312-13
GGTATCA32500.022.5424771
Produced by FastQC (version 0.11.5)