FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18168211
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1056860.5817083476188162No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT433900.23882373448877275No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC402570.22157932886182355No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT365210.20101593932391032No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG356510.19622735557177315No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT185210.10194179272796865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT22700.035.9698265
TGCGACA22450.035.5245554
CCGACTC109500.033.5987665
CGACTCC109900.033.475196
ACCCGAC114250.032.5758363
CGGCCTA58800.032.050579
CTGCGAC25700.031.4015853
CTCACGT338300.031.3475639
CCGACTG147950.030.12828
AACCCGA126350.030.0587432
GCTCACG355750.029.8366098
CGACTGA150800.029.6217619
GCTCCCG137750.028.8096038
TTGGACG31450.028.6782057
CCCGACT131100.028.4975664
TCAGACG34750.028.2781685
ACGTCGT41200.027.6524918-19
ACCGACT162100.027.3516067
GACCGAC162000.027.339196
GCTGCGA32150.025.6935632
Produced by FastQC (version 0.11.5)