FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18168211
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG356570.19626038028730514No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA314360.1730274929105568No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG220500.12136582958002853No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA216000.11888897591513001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA380750.044.4894879
TAGTAGC387100.043.229787
AGTAGCA397850.042.492118
CAGTACG98250.042.3874668
GTAGTAG304100.036.3471266
GTACACC82550.035.7633367
AGTACGA120550.034.4687659
TTCGACG5950.034.3835371
GGTAGTA249900.034.0956045
CGAGAAC323750.033.7297324-25
GGCGGTA74700.033.5014953
CGTCGGT36500.033.4750869
TCAGTCG310500.033.4315584-85
CGAAGTG317750.033.39492440-41
CGCGACA42800.033.3281660-61
AAACGAG335000.033.30028522-23
ACGAGAA332300.033.29812224-25
AAGGCCG331650.033.2448836-37
CAGTCGG311950.033.13918784-85
CAAACGA332800.033.01508320-21
Produced by FastQC (version 0.11.5)