FastQCFastQC Report
Thu 12 May 2022
EGAF00004913616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33204785
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG4123451.2418240322893221TruSeq Adapter, Index 3 (100% over 49bp)
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3643681.0973358207258381No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2667960.8034866059214055No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1732990.5219097187348148No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1193920.3595626353249991No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC753220.22684080020394648No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG740550.223025085089393No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT537700.16193449227272516No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC532340.1603202670940348No Hit
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT488540.14712939716369192TruSeq Adapter, Index 3 (100% over 43bp)
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC480970.1448496052602057No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA404550.1218348500073107No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC386720.11646514199685376No Hit
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT374470.11277591467615286TruSeq Adapter, Index 3 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC345840.10415366339520042TruSeq Adapter, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)