FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004913618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38022389
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG4927641.295983795231804TruSeq Adapter, Index 3 (100% over 49bp)
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4424321.163609156699754No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3160450.8312076340074266No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2125020.558886502371011No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1330410.349901738157484No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC903900.2377283552593184No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG874650.23003551933572614No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC652220.17153577593454214No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT626000.16463983891175277No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC561990.1478050208786197No Hit
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT557670.14666884818836606TruSeq Adapter, Index 3 (100% over 43bp)
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA472740.12433200870150479No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC424230.11157373620053175No Hit
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT411430.10820729859978023TruSeq Adapter, Index 3 (100% over 43bp)
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC402030.10573507098672838No Hit
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC388610.10220557156469048TruSeq Adapter, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)