FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19410709
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2357481.2145254457217405No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1935260.9970063432510374No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1408560.7256612831607543No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1056280.5441738372359299No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1019060.5249988550134876No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT859190.4426371030548137No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC785290.4045653355578099No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC774390.39894987864688514No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA772670.3980637698499318No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG630590.32486706178532687No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA625140.3220593333298645No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA531500.2738179218492225No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT526110.27104110416574684No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC484360.2495323586583056No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC477770.24613732553509507No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC436230.2247367677296074No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT434260.22372186404937605No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG429130.2210789930445096No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT425980.21945617751520566No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG394020.20299103963693443TruSeq Adapter, Index 7 (100% over 49bp)
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG372930.19212590328359463No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG363110.1870668402684312No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG347820.17918974520714312No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG347420.17898367339389817No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC329670.1698392366811537No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT324810.1673354641502276No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA324260.16705211540701578No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA313560.16153969440271349No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG305990.15763978533705286No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC304170.15670215858678835No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT303930.1565785154988414No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC282490.14553306630891225No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA276540.14246774808689366No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC269700.1389439200804051No Hit
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT259750.13381788372593706TruSeq Adapter, Index 7 (100% over 43bp)
AGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTA254280.13099985167981243No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT252760.13021677878948162No Hit
GAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACA251870.12975826900501164No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG244280.12584805634868876No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG237680.12244787143014715No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA235850.12150509288455151No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG231500.11926406191551271No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC229320.11814097053332777No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG224480.1156475015930639No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT223670.1152302061712429No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC220190.11343738139601187No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT219930.11330343471740265No Hit
GTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATCTTTGAGACAAGC218540.11258733516637646No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC216680.11162910123478746No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG215320.11092845706975464No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT212940.10970232978094721No Hit
CACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC210370.10837831838084844No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC209290.10782192448508708No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA206580.10642578795035257No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG205350.10579211712462436No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG204610.1054108842701212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGC21400.075.069351
AGTAGCA67400.052.05058
GGTGTCT10150.050.0445677
GTAGCAA70800.049.8188449
TGTGGTG19250.048.8305444
CTATGCG4450.048.0631521
ATGTGGC6850.047.8760451
CTAGGCC9750.047.2853781
ACACACC169200.046.521972
CGGCCTA174300.045.592799
GTGTGGT14550.045.3533023
TGCTAGT4400.045.3162274
TGCGGCG38000.044.8487246
CAATGGT4350.044.7975581
ATCGCTG33450.043.711317
CACACCC185350.043.6705743
TACAGGC41800.041.161581
ATAGCCC8550.041.136311
TAGTTTG23900.041.1160747
ACTTGGC6000.040.399751
Produced by FastQC (version 0.11.5)