FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19410709
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1660900.8556616865463286No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1498720.7721098698661651No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1007160.5188682185694505No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG882280.4545325984743782No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA801660.41299882451485925No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG734690.37849725118232413No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG721360.3716299080059363No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG605110.3117402872816238No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC547980.2823080805549143No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC501160.25818737481459336No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC484030.24936234941237848No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG459620.23678681700910562No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG391210.2015433851488887No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG387710.19974025678299542No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG365150.18811780651598042No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC361020.18599011504422636Illumina Single End PCR Primer 1 (100% over 50bp)
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG356680.18375423587051867No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA347080.17880851235263998No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG346360.17843758308879906No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC345780.1781387789595939No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG345480.1779842250996602No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC331590.17082838138472944No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA328270.16911798533479638No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG320770.16525413883645362No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA295420.15219433767205515No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC285750.1472125515868586No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG276690.14254502501686053No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA276680.14253987322152942No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA269600.13889240212709386No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG260040.13396728579053965No Hit
GGGCATTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT257590.13270509593441435No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG256870.13233416667057346No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG253360.13052588650934904No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA251040.12933066999252835No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT240290.12379249001157043No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTT233240.12016047430312823No Hit
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG233230.12015532250779712Illumina Single End PCR Primer 1 (100% over 44bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT232090.11956801784004902No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA224180.1154929477331302No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA224170.11548779593779908No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA224110.11545688516581233No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT222720.11474078561478614No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC222670.11471502663813052No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC217850.11223186128852891No Hit
GGGCGCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT215610.11107785913435722No Hit
CCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGT213060.10976415132492069No Hit
GGGCCCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT212440.10944474001439103No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG211270.10884197996064955No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT211020.10871318507737146No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC208120.1072191644313456No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC204560.10538512529346558No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG200780.10343774665830083No Hit
GGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAT196980.10148006443247384No Hit
GCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTG196170.10106276901065284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)