FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004913626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23177423
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5195812.2417548318464915No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3465821.4953431190344155No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2992371.2910710565190961No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1619710.6988309269757902No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG1380350.5955580135030543No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1123820.4848770288224019No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC949750.4097737699311955No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC872610.3764913812894557No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG715530.30871853182297276No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA647850.27951770134237963No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA630800.2721614046565919No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT557090.24035890443903102No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC507620.21901485769146986No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC458550.19784339268433768No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC377910.16305091381384376No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA377120.16271006487649642No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC366550.1581495923856591No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG352840.15223435323245385No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG340550.14693177925777168No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC339770.14659524486393505No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG324780.14012774414135687No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA319930.1380351905386548No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC316490.13655098757096507No Hit
CAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCC311500.13439803035911285No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC310370.13391048694240082No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG309990.13374653428899322No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC309900.13370770339739668No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC303950.13114055000851474No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA303350.1308816773978712No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT287190.12390937508453809No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT286680.12368933336549105No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT283810.12245105937791273No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC281280.12135947986969906No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA275630.11892176278613889No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT272310.1174893343405779No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC256730.11076727555086689No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT256310.1105860647234164No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC256230.11055154837533059No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC255860.11039191026543374No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC250920.10826052577113514No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA250870.1082389530535815No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT250180.1079412495513414No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG248000.10700067906600315No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT247640.10684535549961702No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC238530.10291480636134569No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG234190.10104229447769064No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT233650.10080930912811145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)