FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16966242
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG707050.4167393109210631No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC493640.2909542372435805No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG398980.23516109224423418No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA378560.22312542754017065No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG300200.17693959569832848No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC288440.17000818448776106No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG269960.1591159668711551No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG263020.155025491207776No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261780.15429462812094746No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA220550.12999343048389855No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA180050.1061224990189342No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG179130.10558024576096464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG232350.052.6000028
GGTGTTA110250.049.051762
GATAGTA94550.047.235145
TAGTAGC794800.046.3791247
AGTAGCA803050.046.083188
CGTCGGT85400.045.8127449
GTAGCAA815250.045.5237169
TCAGTAC278700.044.0256357
GTGCTAG70100.043.3566363
AGTACGA293850.041.576879
TGCTAGT79000.040.1580624
TGATCGT43850.038.939115
GTAGTAG605300.038.036316
TTCAGTA251300.037.8348586
GTACGAA188150.036.6599210-11
TAAGCGT70850.036.6533745
GTACACC71900.036.4315227
GCTCCGT43700.036.1350061
ACGAATA192050.036.0592112-13
TGAGAGA677950.035.5948794-95
Produced by FastQC (version 0.11.5)