FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18782437
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG194293410.344419097479204No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT8847614.7105761621881115No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG8012994.2662142298148No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT5308002.8260443519656153No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG4085412.175122429533505No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA3685521.9622160851650934No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3664111.950817138372406No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC2653551.4127825904593743No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA2535251.349798218410103No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1785370.9505529021606727No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1656430.8819036635128871No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1624040.8646588299484247No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG1476540.7861280194896967No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG1361210.7247249118950858No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT1297080.6905813127444538No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA1218970.6489945899991573No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT1095210.5831032469322271No Hit
CCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG1061270.5650331743426054No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC929020.49462165106689826No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC868660.4624852461903639No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG805890.429065727732775No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG671520.35752549043555953No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG667250.3552520900243136No Hit
GAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACA553110.2944825530361156No Hit
TCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTG548870.2922251249930986No Hit
GGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCT540540.2877901307482091No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC507480.2701885809599681No Hit
TCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG484570.25799101575583616No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG478720.25487640395120187No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA476290.2535826421246615No Hit
CCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGC469200.24980783909989956No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA461360.24563372686941531No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG458180.24394065583715255No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT392570.20900908652056174No Hit
GACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATT373040.19861107480355186No Hit
GCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGC366810.1952941463346849No Hit
CCCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG363610.19359042705693622No Hit
TACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTA354120.1885378345738628No Hit
GGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAAC354020.18848459334643317No Hit
CACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG353360.1881332012453975No Hit
CCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCG352870.18787231923099224No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA346220.18433177760692077No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA343490.18287829209809142No Hit
CCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCAC340830.18146207544846282No Hit
ACCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG329550.17545646499439876No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG323260.172107591789074No Hit
CCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACC315190.16781102473550158No Hit
ACGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA313380.16684735851902496No Hit
CAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATC310590.16536192827373786No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA309050.16454201337132132No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT300080.15976627527088205No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG299790.1596118757113361No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC298480.1589144156320077No Hit
ACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGG298360.15885052615909212No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA296620.1579241288018163No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC294370.15672620118464925No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA293480.15625235426052542No Hit
TCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCT280470.14932567057192844No Hit
AACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCC276810.1473770416480034No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACA270400.14396427896976308No Hit
AACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG267850.1426066276703071No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC261170.13905011367800674No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG260950.13893298297766152No Hit
CCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAAT258610.1376871382558078No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA254000.13523271767130113No Hit
TCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG253650.13504637337529737No Hit
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC251830.1340773830360778No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC248440.1322725054262128No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC237780.12659699058221252No Hit
GATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTA234990.1251115603369254No Hit
TCCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG234340.1247654923586327No Hit
ACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGC231810.12341848930466263No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA231410.12320552439494406No Hit
CTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGA231140.12306177308088401No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGT229400.12213537572360816No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG228350.12157634283559689No Hit
TCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCAC225260.11993118890802083No Hit
TCCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA220610.11745547183254228No Hit
GCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTC218130.11613508939228706No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC218030.11608184816485742No Hit
ACCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA214870.1143994253780806No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG214810.11436748064162282No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT214750.11433553590516501No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGG214010.11394155082218566No Hit
TCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGC212360.11306307056959648No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC209570.11157764032430936No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC209320.11144453725573523No Hit
TTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA205950.10965030789135616No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG205580.10945331534986646No Hit
TCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGA202100.1076005206353148No Hit
CATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGG198250.10555073337927341No Hit
CCCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA197910.10536971320601263No Hit
GCCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA195190.10392155181992624No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG194370.10348497375500315No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC194230.10341043603660165No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC193680.10311760928573858No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG191850.10214329482377607No Hit
AGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCC190580.10146713123541956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA9650.083.6569754
GCGACAT10150.079.992945
AACCGCA41050.067.915361
ATAACCG4650.067.411511
CTGCGAC12400.066.2529143
CACGAAT602950.064.415985
CATCACG602550.064.413012
TCACGAA600350.064.411074
GCCGCAC27350.064.078461
ATCACGA606150.063.96713
GCTGCGA12850.063.9334532
AATGGGG613150.063.37879
CCATCAC628350.061.768891
GAATGGG633600.061.3484348
GGGCTCC3186750.060.871436
TCCGCAC39250.060.8655241
GGCTCCC3219900.060.2622687
CTCCCGC3235800.059.924759
TAAACCG4600.059.8843271
CCCGGGG3288600.058.9334752
Produced by FastQC (version 0.11.5)