FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004913633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18782437
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG7478663.9817303792899716No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA3906842.080049569712386No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3747051.9949754124025547No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3383511.801422254204819No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2561841.363955060783646No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1895281.0090703352285968No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1870060.99564289767084No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1763860.9391007141405558No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1500600.7989378588092696No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1481880.7889711010344398No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1446200.769974631087542No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1362520.7254223719744142No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1299900.6920827153579697No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT1142840.6084620435569676No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA997430.5310439747515192No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA972800.5179306604355974No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA968790.5157956872156686No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA937870.4993334996944219No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG915790.4875778366779561No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA845420.45011198493571414No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA826690.4401399030381415No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA816770.4348583732771205No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA753410.4011247315776968No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG697730.3714800161448698No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA676110.3599692627745803No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG646170.3440288392821443No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN607230.32329670532104005No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG596910.3178022106503006No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC576830.3071113721824276No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG546100.29075034299329744No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA536480.28562853691456547No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG530780.2825937869510756No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA520260.27699280982547686No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA485850.2586725034669356No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA479460.25527038903418126No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC472940.2517990610057683No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG453580.24149155937538883No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA450850.2400380738665595No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG444330.23656674583814657No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC435460.23184424896513695No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG432490.23026298451047644No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA403940.21506261407931251No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG399070.21246976630348874No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA384490.2047071953442463No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG381230.20297153133003987No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA378000.2012518396840623No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA371960.19803606954731165No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT329260.1753020654348528No Hit
TGGCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG324780.17291685844600466No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA322940.17193721986129915No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA322520.17171360670609465No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG321020.17091498829464993No Hit
TTGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA316210.16835408525528395No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG314910.16766194929869857No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA312460.16635753922667224No Hit
GGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA295480.15731717880911833No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC295090.1571095380221427No Hit
TGGCCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG287950.15330811438366598No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA285970.15225393808055898No Hit
TGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG285600.1520569455390693No Hit
TGGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA285560.15203564904809744No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG282380.15034257801583467No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG281050.1496344696910204No Hit
GGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG280440.14930969820369955No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC263210.14013623471757153No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC262470.13974224963459214No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC258990.13788945492004046No Hit
TGGTCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA258740.13775635185146634No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG257160.13691514045807793No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA256000.13629754221989404No Hit
TCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG251250.13376858391698584No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT246920.13146323876928218No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC246880.13144194227831033No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG246330.13114911552744726No Hit
TGGTAATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG245900.13092017824949978No Hit
TGGCTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG239480.1275020914485165No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA239480.1275020914485165No Hit
TGGTCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA235260.12525531165098544No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT234640.12492521604092162No Hit
CGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA228970.1219064384456607No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC226090.12037309109568688No Hit
GATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGT222180.11829135910318773No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT221100.11771635384694755No Hit
GGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG218880.11653439859800942No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT209790.11169477102465457No Hit
TGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA208680.1111037934001855No Hit
TGGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA207990.11073642893092094No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA206830.11011883069273706No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA203870.10854289036081953No Hit
GGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG201340.10719588730684947No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC199730.10633870354523217No Hit
TGGCGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG194240.1034157601593446No Hit
GGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA190530.10144051062170474No Hit
TGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG190040.10117962860729947No Hit
GGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA189800.10105184966146831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)