FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946255
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461780.6647898759835336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253980.3656358714156045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209310.3013278378061272No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112530.16200096310889825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77300.073.595681
GTATCAA197350.060.5782471
ATCAACG248450.050.441753
CAACGCA249100.050.2145735
TCAACGC251250.049.855934
AACGCAG260800.047.980186
ACGCAGA288900.043.3320857
CGCAGAG289000.043.2759138
TATCAAC291900.042.8929142
GCAGAGT317800.039.2979559
TCTGTCG10850.037.8382238
CGCTCTG10450.031.8891185
CTCGCTC10150.031.6590963
GTCTCGC9300.030.7137051
TAGCGCG504.4414762E-429.74961924-25
CGGTATC2252.055458E-1029.0927032
GTACTTT453300.028.8415261
AGAGTAC337150.028.4922210-11
GCAGCGC7850.027.2862728
CAGAGTA345950.026.8645210-11
Produced by FastQC (version 0.11.5)