FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946255
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA39050.044.3511625
CGCAGAG42100.041.4043968
ACGCAGA43850.039.6131867
TCTGTCG11300.037.380138
AACGCAG47400.036.770556
TCAACGC47750.036.6440854
ATCAACG49200.035.8065763
GCAGCGC6200.035.5034838
GTCTCGC10500.034.031411
CTCGCTC10200.033.2591323
GTATCAA62600.030.0628931
CGCTCTG11200.029.7579545
GCAGAGT64000.027.8902429
TCTCGCT13200.026.6018092
GGTATCA29350.026.378691
GTACGGG63700.023.7471941
GAGTACG49500.022.9797971
GTCGTGC6800.022.7710171
TGCAGCG10300.021.9468847
CGGATGC13100.020.8928439
Produced by FastQC (version 0.11.5)