FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12079735
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC486530.40276545801708397No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC473580.392045024166507No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG134040.11096269909894546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA68400.073.5372544
GCGACAT70650.071.329755
CTGCGAC79750.063.0803263
GCTGCGA84400.059.5555462
CGACATC86400.058.326946
GGCTGCG111350.045.6335641
CGCACTA34350.040.7908557
TCGACCG16900.040.757733
ACATCTG140000.036.097838
GACATCT144750.034.9132737
GTCCGCA41100.033.8604974
TTGATCG77350.032.6696822-23
CATCTGT159650.032.101119
GCTCGCG21850.031.5198449
TGATCGC83800.030.38192424-25
CTAGGCG155550.029.5883877
TAGGCGG156500.029.2266798
TCCGCAC48050.028.864035
ATTCGGC89000.028.41823838-39
TGATTCG89100.028.3596936-37
Produced by FastQC (version 0.11.5)