FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12079735
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG144930.11997779752618745No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA125410.10381850264099338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG30300.036.8600237
TCGGGGA114000.035.9636967
CGGGGAT113700.035.8975648
TGTCGGG28100.032.523665
CGCGCGT35400.031.5538738
CCCCGTT38100.029.9061362
ATCGGGG84250.029.8050696
TTCCGAC26100.029.6149448
TATCGGG67800.029.599475
GCGCGTC37850.029.3961649
TCTATCG60100.029.1475053
TCCGACC26700.028.2522649
CTCCGTT37100.027.79332
CCGTTTA21450.027.6710514
CCTCGCG36800.027.6509425
CCCGTTA14650.027.6435453
GTCCCGT20050.026.5298611
CTGTCGG33300.026.4532934
TCCGTTT44700.026.2315983
CCCCCGT33800.025.3469451
Produced by FastQC (version 0.11.5)