FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23853945
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC607500.25467485566852777No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC558170.2339948381703739No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC272580.11427040684465399No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT255470.1070975890989939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT85150.066.4488145
TGCGACA86500.065.352994
CTGCGAC93450.060.89433
CGACATC101850.055.224256
GCTGCGA104500.054.9097862
GGCTGCG128600.044.7903821
ACTCGGG173200.031.802311
CTCGGGA184950.029.7151642
CTAGGCG236100.028.4707987
TTGATCG100450.028.20655622-23
TCGGGAG197400.028.1297873
TAGGCGG244550.027.4287628
TGATCGC103000.027.32328824-25
GACATCT218900.026.1722327
GATCGCC109450.025.73479824-25
GCTAGGC265150.025.6023466
TCGCCAG116600.024.48261326-27
GGCTAGG282650.024.4049425
ATTCGGC114850.024.33895138-39
TGATTCG117700.023.95128436-37
Produced by FastQC (version 0.11.5)