FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23853945
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG265790.11142391751133827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC94650.051.0824179
TTCCGAC100250.048.1813558
TGTCGGG43950.037.7116285
TTTCCGA129400.037.5834247
CGGGGAT201150.037.424138
GTTCCGT35150.037.278441
TCGGGGA209500.036.1128967
GTTTCCG115400.035.392076
CCCCCGT43050.033.8562741
GTCCCGT33900.033.6113131
CCGTTTC132450.033.0571144
CGTTTCC127000.032.2712555
CCCCGTT61750.031.8297182
TCCGTTT138000.031.4496443
GCTCCGT85900.031.4480021
CTCCGTT102450.031.186922
ATAGCGC166800.031.0279694-95
GTCGGGG56600.030.709876
CCGATCG179150.030.51005786-87
TCACCCC314200.030.4739449
Produced by FastQC (version 0.11.5)