FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22985833
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC567630.24694776125798878No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT537990.23405286203897852No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT490080.21320958870622614No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC351130.1527593104848539No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC322690.14038647196296952No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT266380.1158887737503357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA84400.069.9043354
GCGACAT86700.068.272475
CTGCGAC91950.064.41793
GCTGCGA100500.058.9848372
CGACATC103750.056.9595646
GGCTGCG132300.045.3660471
ACTCGGG296300.036.8718381
CTCGGGA310750.035.202592
TCGGGAG328300.033.3062933
TTGATCG95600.031.17741422-23
TGATCGC100300.029.47948524-25
GACATCT208750.028.605087
ATTCGGC104500.028.36333738-39
TCGCCAG105700.028.15318726-27
GATCGCC107750.027.70571924-25
TGATTCG108400.027.64949436-37
TTCGGCT108550.027.41437540-41
GGTGTGC166400.026.4072257
ACGGACC57200.026.243154
TCGGCTG114450.026.04264340-41
Produced by FastQC (version 0.11.5)