FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23540798
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT278640.11836472153577801No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC273010.11597312886334608No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT266350.11314399792224546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA57650.054.935024
GCGACAT64100.049.483415
CTGCGAC67450.047.6562543
GCTGCGA81500.039.4988372
CGACATC88950.035.4977956
ACTCGGG222950.035.107391
CTCGGGA263400.032.002812
TCGGGAG277800.030.241523
GGCTGCG108150.027.153421
TAGGCGG148350.026.4615868
CTAGGCG148000.026.4600947
TTGATCG69400.023.08955422-23
GCTAGGC174000.022.6425256
CGGGAGG406950.021.5760574
TCGCACG33650.021.13338942-43
GCGCGGT55700.020.7686236
GGCTAGG196700.020.6811395
TGATCGC77450.020.4752124-25
ACGGACC48950.019.7583054
TGGCTAG213000.019.1199054
Produced by FastQC (version 0.11.5)