FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23540798
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG302640.1285597879901947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC134400.056.0485469
TTCCGAC138100.054.7488148
TTTCCGA163900.046.101477
GTTTCCG145950.044.6777086
CGTTTCC150000.041.2592165
CCGTTTC156400.040.4511344
GTTCCGT43950.038.0336951
TCCGTTT164550.036.134163
GCTCCGT121800.035.9864651
CTCCGTT129500.034.982992
TCACCCC447900.034.8879369
ATAGCGC246000.034.3930994-95
GTTCACC456900.034.2601747
CGGGGAT126950.034.0060778
GTCCCGT36600.032.696721
CCGATCG272650.032.13551786-87
TTCACCC488250.031.9535058
CGATCGG272200.031.4469786-87
GATCGGC282150.031.18223288-89
CCCCGTT68850.031.1754532
Produced by FastQC (version 0.11.5)